'Shotgun DNA synthesis' for the high-throughput construction of large DNA molecules.

Publication Date: 2012 Jun 16 PMID: 22705793
Authors: Kim, H. - Han, H. - Ahn, J. - Lee, J. - Cho, N. - Jang, H. - Kim, H. - Kwon, S. - Bang, D.
Journal: Nucleic Acids Res

We developed a highly scalable 'shotgun' DNA synthesis technology by utilizing microchip oligonucleotides, shotgun assembly and next-generation sequencing technology. A pool of microchip oligonucleotides targeting a penicillin biosynthetic gene cluster were assembled into numerous random fragments, and tagged with 20 bp degenerate barcode primer pairs. An optimal set of error-free fragments were identified by high-throughput DNA sequencing, selectively amplified using the barcode sequences, and successfully assembled into the target gene cluster.

(Via Nucleic Acids Research.)