Methods Mol Biol. 2012;852:11-21
Authors: Gibson DG
Abstract
The yeast Saccharomyces cerevisiae can take up and assemble at least 38 overlapping single-stranded oligonucleotides and a linear double-stranded vector in one transformation event. These oligonucleotides can overlap by as few as 20 bp and can be as long as 200 nucleotides in length to produce kilobase-sized synthetic DNA molecules. A protocol for designing the oligonucleotides to be assembled, transforming them into yeast, and confirming their assembly is described here. This straightforward scheme for assembling chemically synthesized oligonucleotides can be a useful tool for building synthetic DNA molecules.
PMID: 22328422 [PubMed - in process]
Oligonucleotide assembly in yeast to produce synthetic DNA fragments.
(Via pubmed: "synthetic biology".)